Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

ACMATE506 Mapping and Delivery Guide
Assist to collect and transfer embryos of mice

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency ACMATE506 - Assist to collect and transfer embryos of mice
Description
Employability Skills
Learning Outcomes and Application This unit of competency describes the skills and knowledge required to assist in the collection, handling, storage and transfer of mouse embryos.The unit applies to individuals who work as animal technicians in research and teaching facilities that require laboratory/research mice to be re-derived by embryo transfer for scientific purposes. All work practices must be undertaken in accordance with legislative requirements, the current Australian Code for the Care and Use of Animals for Scientific Purposes (the Code), the organisation's standard operating procedures, and protocols, policies and procedures approved by the organisation's Animal Ethics Committee (AEC). Users are advised to check legislative requirements for their jurisdiction.
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.
Prerequisites/co-requisites
Competency Field
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities
Slides
PPT
Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Prepare for procedures and maintain documentation
  • Comply with organisational protocols, policies and procedures as set down by the AEC and relevant legislative requirements, including work health and safety, at all times
  • Identify regulatory and project documentation requirements
  • Verify project reproductive and breeding program objectives against instructions
  • Maintain and update records according to organisational policies and procedures
       
Element: Assist to vasectomise males
  • Prepare equipment and work areas
  • Select male mice of appropriate age for vasectomy and assess for health status and suitability for surgery
  • Prepare and verify contingency plans to respond to potential emergencies during and after surgery
  • Prepare anaesthetics, analgesics and equipment according to instructions or project specifications
  • Anaesthetise animal under supervision and monitor to ensure surgical anaesthesia is achieved and maintained
  • Prepare animal for aseptic surgery
  • Conduct vasectomy and close wounds under supervision
  • Monitor animal during recovery
  • Confirm success of vasectomy
       
Element: Assist to collect embryos
  • Monitor females of appropriate age for stages of oestrus cycle
  • Identify suitable females and assess health status
  • Prepare female for super-ovulation according to organisational procedure
  • Mate female with an entire male
  • Confirm presence or absence of vaginal plugs
  • Collect embryos under supervision from the reproductive tract of super-ovulated and mated donor females
       
Element: Manage and manipulate embryos
  • Hold embryos in a suitable receptacle under optimal temperature and atmospheric conditions to ensure viability
  • Grade and manipulate embryos
  • Wash embryos in an aseptic manner for the purpose of re-derivation transfer
  • Store embryos before transfer
  • Monitor procedures, materials, equipment and techniques for quality
       
Element: Assist to transfer embryos
  • Mate females with vasectomised males and check for the presence of vaginal plugs
  • Prepare embryos for transfer
  • Under supervision, anaesthetise females for embryo transfer and monitor to ensure surgical anaesthesia is achieved and maintained
  • Transfer embryos under supervision
       
Element: Monitor success of embryo transfer
  • Monitor females for pregnancy using appropriate technique
  • Monitor and record number of pups born to embryos transferred per recipient to determine success of program
  • Assess health status of pups and recipient after birth and weaning
       

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)


Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Comply with organisational protocols, policies and procedures as set down by the AEC and relevant legislative requirements, including work health and safety, at all times 
Identify regulatory and project documentation requirements 
Verify project reproductive and breeding program objectives against instructions 
Maintain and update records according to organisational policies and procedures 
Prepare equipment and work areas 
Select male mice of appropriate age for vasectomy and assess for health status and suitability for surgery 
Prepare and verify contingency plans to respond to potential emergencies during and after surgery 
Prepare anaesthetics, analgesics and equipment according to instructions or project specifications 
Anaesthetise animal under supervision and monitor to ensure surgical anaesthesia is achieved and maintained 
Prepare animal for aseptic surgery 
Conduct vasectomy and close wounds under supervision 
Monitor animal during recovery 
Confirm success of vasectomy 
Monitor females of appropriate age for stages of oestrus cycle 
Identify suitable females and assess health status 
Prepare female for super-ovulation according to organisational procedure 
Mate female with an entire male 
Confirm presence or absence of vaginal plugs 
Collect embryos under supervision from the reproductive tract of super-ovulated and mated donor females 
Hold embryos in a suitable receptacle under optimal temperature and atmospheric conditions to ensure viability 
Grade and manipulate embryos 
Wash embryos in an aseptic manner for the purpose of re-derivation transfer 
Store embryos before transfer 
Monitor procedures, materials, equipment and techniques for quality 
Mate females with vasectomised males and check for the presence of vaginal plugs 
Prepare embryos for transfer 
Under supervision, anaesthetise females for embryo transfer and monitor to ensure surgical anaesthesia is achieved and maintained 
Transfer embryos under supervision 
Monitor females for pregnancy using appropriate technique 
Monitor and record number of pups born to embryos transferred per recipient to determine success of program 
Assess health status of pups and recipient after birth and weaning 

Forms

Assessment Cover Sheet

ACMATE506 - Assist to collect and transfer embryos of mice
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:


Assessment Record Sheet

ACMATE506 - Assist to collect and transfer embryos of mice

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: